Zearalenone fluorescence quantitative test strip performance evaluation test

The performance of zearalenone fluorescent quantitative test strips developed and produced by Shanghai Feibi Biotechnology Co., Ltd. was evaluated in grain and grain feeds. The product had a limit of detection (LOD) of 0.29 μg/kg, a limit of quantification (LOQ) of 0.91 μg/kg, a linear range of 1.00 to 50.00 μg/kg, and a linear recovery range of 92.89% to 121.07. %, the coefficient of variation for 3 replicates is within 14.56%. The cross-reactivity with other mycotoxins was less than 5%. The product has the advantages of easy operation, sensitive and accurate, rapid quantification, good reproducibility, etc. It is suitable for the rapid quantitative determination of zearalenone in grain and grain feed.

Key words: zearalenone test strip; fluorescence quantitative immunochromatography; zearalenone; grain and grain feed

Zearalenone (ZEN), also known as F-2 toxin, is mainly produced by Fusarium graminearum. It was first isolated from corn with head blight. It is a lactone structure of dihydroxybenzoic acid with a molecular formula of C18H22O5. Zearalenone often contaminates corn, wheat, rice, barley, millet, and oats. Zearalenone has the effect of estrogen and its intensity is one-tenth that of estrogen and can result in increased levels of estrogen in poultry and livestock. It has been found that pigs are more sensitive to this toxin. The target organs for the action of zearalenone are mainly the reproductive system of female animals, and also have a certain influence on male animals, which can cause females, poultry and experimental mice to produce female hyperthyroidism. Foods containing zearalenone during pregnancy (including humans) can cause miscarriage, stillbirths and teratogenicity. Because of the above hazards, zearalenone has been identified by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO) as one of the most dangerous naturally occurring food contaminants. The Chinese national standard GB 2761-2011 (Limit of mycotoxins in food) has a maximum requirement of 60 μg/kg for ZEN in grains and its products. GB 13078.2-2006 "Permissible Amount of Ochratoxin A and Zearalenone in Feed Hygiene Standard Feeds", the maximum limit for zearalenone in different feeds is 500 μg/kg

At present, the HPLC method for the determination of zearalenone by the national standard method requires large-scale equipment and equipment, which is expensive, the operation process is complicated, and the time is long, and it cannot be used in the field and basic small laboratory. In addition, zearalenone enzyme immunoassay kits or colloidal gold rapid test strips are also used for screening of large numbers of samples. The operation is simple, the detection is rapid, and the cost is low, but the accuracy and repeatability Poor, easy to cause false positive or false negative. Therefore, there is an urgent need for a detection method that is both simple and rapid and that can be accurately quantified. Fluorescence quantitative immunochromatography precisely meets this need. In this paper, the performance of a variety of grain and grain feed samples was tested to verify the technical performance of the zearalenone fluorescent quantitative test strip developed by Shanghai FeiDian.

In this experiment, the zearalenone fluorescent quantitative test strips produced by Shanghai Feixie Biotechnology Co., Ltd. were used to test different grain and grain feed samples. The lowest detection limit LOD of the product was 3.65 μg/kg, the lowest quantitative The limit LOQ was 8.51μg/kg, the linear range was 10.00-1000.00μg/kg, the addition recovery was linear in the range of 94.00% to 118.33%, and the coefficient of variation for three replicates was within 13.11%. The cross-reactivity with other mycotoxins was less than 5%. Its accuracy and reliability can meet the technical requirements of the analysis standard of zearalenone in EU and China. The method is simple, rapid, accurate, reliable, and reproducible. It can be used for the rapid quantitative analysis of zearalenone in different grain and grain feeds.